Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Food & Nutrition Science Notes
Introduction of DPR, an Enterostatin Fragment Peptide, into Soybean β-Conglycinin α′ Subunit by Site-directed Mutagenesis
Yasuyuki TAKENAKANaomi DOYAMANobuyuki MARUYAMAShigeru UTSUMIMasaaki YOSHIKAWA
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2004 Volume 68 Issue 1 Pages 253-256

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Abstract

  DPR, a fragment peptide of enterostatin (VPDPR) having hypocholesterolemic activity, was introduced into the three homologous sites, EPR, DYR, and DPI, in the soybean β-conglycinin α′ subunit by site-directed mutagenesis. The modified β-conglycinin was expressed in Escherichia coli and recovered in the soluble fraction. After purification on ion-exchange HPLC, the modified β-conglycinin was digested by trypsin to release integrated DPR. The yield of DPR from 1 mole of the modified β-conglycinin was 1.2 mole.

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© 2004 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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