Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
Genetic Analysis of an Incomplete bio Operon in a Biotin Auxotrophic Strain of Bacillus subtilis Natto OK2
Mayumi SASAKIFujio KAWAMURAYasurou KURUSU
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JOURNAL FREE ACCESS

2004 Volume 68 Issue 3 Pages 739-742

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Abstract

We describe the genetic analysis of the bio operon of the biotin auxotrophic Bacillus subtilis natto OK2 strain. The OK2 strain would only cross-feed with the Escherichia coli bioB mutant and also grew well in medium containing dethiobiotin. Sequencing analysis revealed two significant genetic alterations in the bioW and bioF genes within the bio operon of the OK2 strain. Complementation analysis with B. subtilis 168 bio mutants demonstrated that only the bioB gene could complement, but other bio operon genes could not. A bio+ transformant, isolated from an OK2 strain, has biotin autotrophy.

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© 2004 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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