Functional Analysis of the YvrGHb Two-Component System of Bacillus subtilis: Identification of the Regulated Genes by DNA Microarray and Northern Blot Analyses

Masakuni SERIZAWA; Keisuke KODAMA; Hiroki YAMAMOTO; Kazuo KOBAYASHI; Naotake OGASAWARA; Junichi SEKIGUCHI

doi:10.1271/bbb.69.2155

Biochemistry & Molecular Biology Regular Papers

Functional Analysis of the YvrGHb Two-Component System of Bacillus subtilis: Identification of the Regulated Genes by DNA Microarray and Northern Blot Analyses

Masakuni SERIZAWA, Keisuke KODAMA, Hiroki YAMAMOTO, Kazuo KOBAYASHI, Naotake OGASAWARA, Junichi SEKIGUCHI

Author information

Keywords: Bacillus subtilis, two-component system, cell surface proteins, extracytoplasmic function sigma factor (ECF sigma factor), antibiotic susceptibility

JOURNAL FREE ACCESS

2005 Volume 69 Issue 11 Pages 2155-2169

DOI https://doi.org/10.1271/bbb.69.2155

Details

Abstract

YvrGHb is a novel two-component system in Bacillus subtilis. The yvrG and yvrHb genes are considered to encode sensor kinase and response regulator respectively. We found that the YvrGHb system positively regulates the 7 transcriptional units (wprA, wapA-yxxG, dltABCDE, sunA, sunT-bdbA-yolJ-bdbB, yvrI-yvrHa, and sigX-rsiX), and negatively regulates the lytABC operon. wprA, wapA, lytB, and lytC encode the main cell surface proteins of B. subtilis. Furthermore, SigX [extracytoplasmic function sigma factor (ECF sigma factor)] relating to the cell surface homeostatic functions, was regulated by this system. The yvrGHb null mutant showed the unusual autolysis and higher susceptibility to the four kinds of antibiotics (aztreonam, cefepime, bacitracin, and fosfomycin). These results indicate that the YvrGHb system is important to maintain the overall state of the cell surface through transcriptional regulation.

Corresponding author

Register with J-STAGE for free!