Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
Structural and Functional Analysis of pTB6 from Bifidobacterium longum
Kunihito TANAKAKazuya SAMURAYasunobu KANO
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JOURNAL FREE ACCESS

2005 Volume 69 Issue 2 Pages 422-425

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Abstract

The complete nucleotide sequence for pTB6 (3,624 bp) from Bifidobacterium longum was determined. This plasmid is 95% homologous in nucleotide (nuc) sequence, and also 92% in RepB aa sequence, to rolling circle replication (RCR) plasmids pKJ36 and pB44, suggesting that pTB6 replicates by the rolling circle mechanism. The putative MembB, MobA, and protein encoding from orf (Orf) I detected were nonessential for plasmid replication. We constructed an immobile shuttle vector from pTB6 and pUC18, which transformed B. longum with a high efficiency of 2.5×106 transformants/μg DNA.

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© 2005 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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