Identification of the Putative Proton Donor Residue of Lacto-N-biose Phosphorylase (EC 2.4.1.211)

Mamoru NISHIMOTO; Motomitsu KITAOKA

doi:10.1271/bbb.70064

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Biochemistry & Molecular Biology Notes

Identification of the Putative Proton Donor Residue of Lacto-N-biose Phosphorylase (EC 2.4.1.211)

Mamoru NISHIMOTO, Motomitsu KITAOKA

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Keywords: lacto-N-biose, lacto-N-biose phosphorylase, phosphorylase, Bifidobacterium

JOURNAL FREE ACCESS

2007 Volume 71 Issue 6 Pages 1587-1591

DOI https://doi.org/10.1271/bbb.70064

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Abstract

Two lacto-N-biose phosphorylase (LNBP) isozyme genes were cloned from Bifidobacterium bifidum JCM1254. Alignment of the amino acid sequences of LNBP and its homologs identified 24 completely conserved acidic amino acid residues. All single mutants of Bifidobacterium longum LNBP at residues other than D313N retained considerable activity, suggesting that Asp313 is the putative proton donor residue in LNBP.

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