Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Microbiology & Fermentation Technology Regular Papers
Identification of the Functional Periplasmic Nitrate Reductase (nap) Gene Cluster from the Deep-Sea Denitrifier Pseudomonas sp. Strain MT-1
Hideyuki TAMEGAIEriko IKEDAChiaki KATOKoki HORIKOSHI
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2007 Volume 71 Issue 8 Pages 2041-2045

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Abstract

The nap gene cluster encoding periplasmic nitrate reductase was identified from Pseudomonas sp. strain MT-1, a deep-sea denitrifier isolated from the Mariana Trench. The ORFs identified were highly homologous with those of Pseudomonas stutzeri, but the cluster included only four ORFs (napDABC), less than those in other organisms. For other bacteria, some additional small ORFs (such as napE, napF, napG, napH, and napK) are found in the nap gene cluster, although their physiological function is still unclear. The soluble fraction of MT-1 grown under denitrifying condition showed significant nitrate reductase activity. This observation suggests that the periplasmic nitrate reductase encoded by the gene cluster identified in this study is functional. The activity was highest when the organism was grown under denitrifying conditions, suggesting that the enzyme participates in dissimilatory nitrite reduction.

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© 2007 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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