Distribution of <small>L</small>-Azetidine-2-carboxylate N-Acetyltransferase in Yeast

Masaru WADA; Kae OKABE; Michihiko KATAOKA; Sakayu SHIMIZU; Atsushi YOKOTA; Hiroshi TAKAGI

doi:10.1271/bbb.70534

Microbiology & Fermentation Technology Note

Distribution of L-Azetidine-2-carboxylate N-Acetyltransferase in Yeast

Masaru WADA, Kae OKABE, Michihiko KATAOKA, Sakayu SHIMIZU, Atsushi YOKOTA, Hiroshi TAKAGI

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Keywords: azetidine-2-carboxylate, MPR1, N-acetyltransferase, proline analog resistance, yeast

JOURNAL FREE ACCESS

2008 Volume 72 Issue 2 Pages 582-586

DOI https://doi.org/10.1271/bbb.70534

View "Advance Publication" version (February 7, 2008).

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Abstract

The budding yeast Saccharomyces cerevisiae Σ1278b contains the MPR1 gene encoding N-acetyltransferase, which detoxifies the L-proline analog L-azetidine-2-carboxylate (AZC). Of 131 yeasts tested, AZC acetyltransferase activity was detected in 17 strains of 41 strains that showed AZC resistance. Degenerate-PCR analysis revealed that two strains, i.e., Candida saitoana AKU4533 and Wickerhamia fluorescens AKU4722, contained a DNA fragment highly homologous to MPR1. This indicates that AZC acetyltransferases are widely distributed in yeasts.

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