Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research

Tsuyoshi NAKAGAWA; Shinya NAKAMURA; Katsunori TANAKA; Makoto KAWAMUKAI; Takamasa SUZUKI; Kenzo NAKAMURA; Tetsuya KIMURA; Sumie ISHIGURO

doi:10.1271/bbb.70678

Biochemistry & Molecular Biology Communication

Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research

Tsuyoshi NAKAGAWA, Shinya NAKAMURA, Katsunori TANAKA, Makoto KAWAMUKAI, Takamasa SUZUKI, Kenzo NAKAMURA, Tetsuya KIMURA, Sumie ISHIGURO

Author information

Tsuyoshi NAKAGAWA
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
Shinya NAKAMURA
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
Katsunori TANAKA
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
Makoto KAWAMUKAI
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
Takamasa SUZUKI
Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
Kenzo NAKAMURA
Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
Tetsuya KIMURA
Department of Sustainable Resource Science, Faculty of Bioresources, Mie University
Sumie ISHIGURO
Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University

Keywords: binary vector, Gateway cloning, promoter swapping, reporter, tag

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Supplementary material

2008 Volume 72 Issue 2 Pages 624-629

DOI https://doi.org/10.1271/bbb.70678

View "Advance Publication" version (February 7, 2008).

Details

Abstract

We developed a new series of Gateway binary vectors, R4pGWBs, that are plant transformation vectors designed for one-step construction of chimeric genes between any promoter and any cDNA. The structure of R4pGWBs is almost the same as the promoterless type of improved pGWBs (ImpGWBs), except that the attR1 site is replaced with attR4, which enables tripartite recombination of these vectors with promoter- and cDNA-entry clones. While ImpGWBs are suitable for promoter analysis and constitutive expression of cDNAs in higher plants, R4pGWBs have a great advantage in expressing a cDNA under the regulation of desired promoters.

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