In Vivo and in Vitro Complementation Study Comparing the Function of DnaK Chaperone Systems from Halophilic Lactic Acid Bacterium Tetragenococcus halophilus and Escherichia coli

Shinya SUGIMOTO; Kozue SARUWATARI; Chihana HIGASHI; Keigo TSURUNO; Shunsuke MATSUMOTO; Jiro NAKAYAMA; Kenji SONOMOTO

doi:10.1271/bbb.70691

Biochemistry & Molecular Biology Regular Papers

In Vivo and in Vitro Complementation Study Comparing the Function of DnaK Chaperone Systems from Halophilic Lactic Acid Bacterium Tetragenococcus halophilus and Escherichia coli

Shinya SUGIMOTO, Kozue SARUWATARI, Chihana HIGASHI, Keigo TSURUNO, Shunsuke MATSUMOTO, Jiro NAKAYAMA, Kenji SONOMOTO

Author information

Shinya SUGIMOTO
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
Laboratory of Functional Food Design, Department of Functional Metabolic Design, Bio-Architecture Center, Kyushu University
Kozue SARUWATARI
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
Chihana HIGASHI
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
Keigo TSURUNO
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
Shunsuke MATSUMOTO
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
Jiro NAKAYAMA
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
Kenji SONOMOTO
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
Laboratory of Functional Food Design, Department of Functional Metabolic Design, Bio-Architecture Center, Kyushu University

Keywords: molecular chaperone, DnaK system, complementation, halophilic lactic acid bacterium, cooperation

JOURNAL FREE ACCESS

2008 Volume 72 Issue 3 Pages 811-822

DOI https://doi.org/10.1271/bbb.70691

View "Advance Publication" version (March 7, 2008).

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Abstract

In this study, we characterized the DnaK chaperone system from Tetragenococcus halophilus, a halophilic lactic acid bacterium. An in vivo complementation test showed that under heat stress conditions, T. halophilus DnaK did not rescue the growth of the Escherichia coli dnaK deletion mutant, whereas T. halophilus DnaJ and GrpE complemented the corresponding mutations of E. coli. Purified T. halophilus DnaK showed intrinsic weak ATPase activity and holding chaperone activity in vitro, but T. halophilus DnaK did not cooperate with the purified DnaJ and GrpE from either T. halophilus or E. coli in ATP hydrolysis or luciferase-refolding reactions under the conditions tested. E. coli DnaK, however, cross-reacted with those from both bacteria. This difference in the cooperation with DnaJ and GrpE appears to result in an inability of T. halophilus DnaK to replace the in vivo function of the DnaK chaperone of E. coli.

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