Biochemistry & Molecular Biology Notes
Restriction on Conjugational Transfer of pLS20 in Bacillus subtilis 168
2008 Volume 72 Issue 9 Pages 2472-2475
Details
2008 Volume 72 Issue 9 Pages 2472-2475
Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking XhoI.
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