Anti-Inflammatory Effect of Buckwheat Sprouts in Lipopolysaccharide-Activated Human Colon Cancer Cells and Mice

Satoshi ISHII; Takafumi KATSUMURA; Chikara SHIOZUKA; Keisuke OOYAUCHI; Kunito KAWASAKI; Shigenobu TAKIGAWA; Tatsunobu FUKUSHIMA; Yoshihiko TOKUJI; Mikio KINOSHITA; Masao OHNISHI; Mika KAWAHARA; Kiyoshi OHBA

doi:10.1271/bbb.80324

Biochemistry & Molecular Biology Regular Papers

Anti-Inflammatory Effect of Buckwheat Sprouts in Lipopolysaccharide-Activated Human Colon Cancer Cells and Mice

Satoshi ISHII, Takafumi KATSUMURA, Chikara SHIOZUKA, Keisuke OOYAUCHI, Kunito KAWASAKI, Shigenobu TAKIGAWA, Tatsunobu FUKUSHIMA, Yoshihiko TOKUJI, Mikio KINOSHITA, Masao OHNISHI, Mika KAWAHARA, Kiyoshi OHBA

Author information

Satoshi ISHII
Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine
Takafumi KATSUMURA
Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine
Chikara SHIOZUKA
Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine
Keisuke OOYAUCHI
Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine
Kunito KAWASAKI
Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine
Shigenobu TAKIGAWA
National Agricultural Research Center for Hokkaido Region
Tatsunobu FUKUSHIMA
Yokohama Research Laboratories, Mitsubishi Rayon Co., Ltd.
Yoshihiko TOKUJI
Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine
Mikio KINOSHITA
Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine
Masao OHNISHI
Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine
Mika KAWAHARA
Hokkaido Tokachi Area Regional Food Processing Technology Center
Kiyoshi OHBA
Hokkaido Tokachi Area Regional Food Processing Technology Center

Keywords: anti-inflammatory effect, buckwheat sprouts, lipopolysaccharide-activated cells, cytokine expression

JOURNAL FREE ACCESS

2008 Volume 72 Issue 12 Pages 3148-3157

DOI https://doi.org/10.1271/bbb.80324

View "Advance Publication" version (December 7, 2008).

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Abstract

In conducting an in vitro screening of ethanol extracts from various natural foods using a human colon cancer cell line (CoLoTC cells), an extract of buckwheat sprouts (ExtBS) was found to express significant anti-inflammatory activity. The anti-inflammatory activity of ExtBS was confirmed by oral administration of lipopolysaccharide (LPS) to mice. Inflammatory cytokines (interleukin 6 and tumor necrosis factor alpha) were markedly up-regulated in the spleen and liver from LPS-administrated mice, and combinatory treatment with LPS and ExtBS decreased up-regulation of them in both cytokines. Both serum cytokine levels corresponded to their gene expressions in tissues, but no anti-inflammatry effect in mice was observed when ExtBS was treated intraperitoneally. ExtBS oral administration also showed protective activity as to hepatic injury induced by galactosamine/LPS treatment. Based on these data, we suggest that ExtBS contains anti-inflammatory compounds.

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