Prenyl Alcohol Production by Expression of Exogenous Isopentenyl Diphosphate Isomerase and Farnesyl Diphosphate Synthase Genes in Escherichia coli

Chikara OHTO; Masayoshi MURAMATSU; Shusei OBATA; Eiji SAKURADANI; Sakayu SHIMIZU

doi:10.1271/bbb.80446

Microbiology & Fermentation Technology Notes

Prenyl Alcohol Production by Expression of Exogenous Isopentenyl Diphosphate Isomerase and Farnesyl Diphosphate Synthase Genes in Escherichia coli

Chikara OHTO, Masayoshi MURAMATSU, Shusei OBATA, Eiji SAKURADANI, Sakayu SHIMIZU

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Keywords: farnesol, geranylgeraniol, lipoate, pyruvate, terpenoid

JOURNAL FREE ACCESS

2009 Volume 73 Issue 1 Pages 186-188

DOI https://doi.org/10.1271/bbb.80446

View "Advance Publication" version (January 7, 2009).

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Abstract

Isopentenyl diphosphate isomerase (idi) and farnesyl diphosphate synthase (ispA) genes were overexpressed in Escherichia coli. The resulting transformant showed 6.8-fold higher production of farnesol (389 μg/l). In a similar manner, overexpression of idi and mutated ispA led to high production of geranylgeraniol (128 μg/l).

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