SK66-his, a Novel Glycine-Rich Peptide Derived from Drosophila with Antibacterial Activity

Zhiguo FENG; Huijuan LIU; Jun LANG; Yanjiao LI; Muya SHU; Zhengwang CHEN

doi:10.1271/bbb.80756

Biochemistry & Molecular Biology Notes

SK₆₆-his, a Novel Glycine-Rich Peptide Derived from Drosophila with Antibacterial Activity

Zhiguo FENG, Huijuan LIU, Jun LANG, Yanjiao LI, Muya SHU, Zhengwang CHEN

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Keywords: antibacterial peptides, glycine-rich, expression, purification, Drosophila

JOURNAL FREE ACCESS

2009 Volume 73 Issue 3 Pages 769-771

DOI https://doi.org/10.1271/bbb.80756

View "Advance Publication" version (March 7, 2009).

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Abstract

SK₆₆-his, a novel glycine-rich peptide derived from the CG13551 gene of Drosophila, was directly expressed in Escherichia coli with the help of the glucose effect of the lac repressor and efficiently purified in high yield (10.063 mg/l). It showed significant activity against Gram-positive bacteria. We determined a MIC value of 9 μg ml⁻¹ with Bacillus thuringiensis.

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