Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
Biotransformation of Daidzein to Equol by Crude Enzyme from Asaccharobacter celatus AHU1763 Required an Anaerobic Environment
Charin THAWORNKUNOMichiko TANAKATeruo SONEKozo ASANO
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JOURNAL FREE ACCESS

2009 Volume 73 Issue 6 Pages 1435-1438

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Abstract

Asaccharobacter celatus AHU1763 is a Gram-positive, obligate anaerobic, non-spore forming, rod-shaped bacteria that was successfully isolated from rat cecal content. Daizein was converted to equol via dihydrodaidzein by this bacterium. A crude enzyme that converted daidzein to dihydrodaidzein was detected mainly in the culture supernatant. The ability of this enzyme dropped after the culture supernatant was exposed to a normal atmospheric environment for even 5 min. Furthermore, the enzyme responsible for changing dihydrodaidzein to equol was detected mainly in the cell debris, which required anaerobic conditions for its activity.

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© 2009 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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