Pinostrobin from Boesenbergia pandurata Is an Inhibitor of Ca2+-Signal-Mediated Cell-Cycle Regulation in the Yeast Saccharomyces cerevisiae

Wachirasak WANGKANGWAN; Saipin BOONKERD; Warinthorn CHAVASIRI; Kasama SUKAPIROM; Kovit PATTANAPANYASAT; Ngampong KONGKATHIP; Tokichi MIYAKAWA; Chulee YOMPAKDEE

doi:10.1271/bbb.90114

Biochemistry & Molecular Biology Notes

Pinostrobin from Boesenbergia pandurata Is an Inhibitor of Ca²⁺-Signal-Mediated Cell-Cycle Regulation in the Yeast Saccharomyces cerevisiae

Wachirasak WANGKANGWAN, Saipin BOONKERD, Warinthorn CHAVASIRI, Kasama SUKAPIROM, Kovit PATTANAPANYASAT, Ngampong KONGKATHIP, Tokichi MIYAKAWA, Chulee YOMPAKDEE

Author information

Wachirasak WANGKANGWAN
Department of Microbiology, Faculty of Science, Chulalongkorn University
Saipin BOONKERD
Program in Biotechnology, Faculty of Science, Chulalongkorn University
Warinthorn CHAVASIRI
Department of Chemistry, Faculty of Science, Chulalongkorn University
Kasama SUKAPIROM
Office for Research and Development, Faculty of Medicine, Siriraj Hospital, Mahidol University
Kovit PATTANAPANYASAT
Office for Research and Development, Faculty of Medicine, Siriraj Hospital, Mahidol University
Ngampong KONGKATHIP
Department of Chemistry, Faculty of Science, Kasetsart University
Tokichi MIYAKAWA
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
Chulee YOMPAKDEE
Department of Microbiology, Faculty of Science, Chulalongkorn University

Keywords: yeast based assay, Boesenbergia pandurata, pinostrobin, Ca²⁺ signal, inhibitor

JOURNAL FREE ACCESS

2009 Volume 73 Issue 7 Pages 1679-1682

DOI https://doi.org/10.1271/bbb.90114

View "Advance Publication" version (July 7, 2009).

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Abstract

Upon searching plant extracts for inhibitors of the Ca²⁺ signaling pathway using the zds1Δ-yeast proliferation based assay, a crude rhizome extract of Boesenbergia pandurata was found to be strongly positive, and from this extract pinostrobin, alpinetin, and pinocembrin chalcone were isolated as active components. Further biochemical experiments confirmed that pinostrobin possesses inhibitory activity on the Ca²⁺ signals involved in the control of G2/M phase cell cycle progression in Saccharomyces cerevisiae.

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