A New Lipid Transfer Protein Homolog Identified as an IgE-Binding Antigen from Japanese Cedar Pollen

Ahmed Ragaa Nour IBRAHIM; Seiji KAWAMOTO; Minori NISHIMURA; Syunka PAK; Tsunehiro AKI; Araceli DIAZ-PERALES; Gabriel SALCEDO; Juan A. ASTURIAS; Takaharu HAYASHI; Kazuhisa ONO

doi:10.1271/bbb.90685

Biochemistry & Molecular Biology Regular Papers

A New Lipid Transfer Protein Homolog Identified as an IgE-Binding Antigen from Japanese Cedar Pollen

Ahmed Ragaa Nour IBRAHIM, Seiji KAWAMOTO, Minori NISHIMURA, Syunka PAK, Tsunehiro AKI, Araceli DIAZ-PERALES, Gabriel SALCEDO, Juan A. ASTURIAS, Takaharu HAYASHI, Kazuhisa ONO

Author information

Ahmed Ragaa Nour IBRAHIM
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
Seiji KAWAMOTO
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
Minori NISHIMURA
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
Syunka PAK
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
Tsunehiro AKI
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
Araceli DIAZ-PERALES
Unidad de Bioquimica, Departamento de Biotecnologia, E.T.S. Ingenieros Agronomos, Universidad Politecnica
Gabriel SALCEDO
Unidad de Bioquimica, Departamento de Biotecnologia, E.T.S. Ingenieros Agronomos, Universidad Politecnica
Juan A. ASTURIAS
Research and Development Department, Bial-Aristegui
Takaharu HAYASHI
Takanobashi Central Hospital
Kazuhisa ONO
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University

Keywords: allergen, lipid transfer protein, Japanese cedar pollen, oral allergy syndrome

JOURNAL FREE ACCESS

2010 Volume 74 Issue 3 Pages 504-509

DOI https://doi.org/10.1271/bbb.90685

View "Advance Publication" version (March 7, 2010).

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Abstract

Japanese cedar (Cryptomeria japonica) pollen is a major cause of seasonal rhinitis and conjunctivitis in Japan, and an understanding of its full allergen repertoire is prerequisite for the development of future molecular diagnostics and immunotherapeutic strategies. Here we report the identification of a new C. japonica pollen IgE-binding antigen (CJP-8) homologous to lipid transfer proteins (LTPs), a class of plant cross-reactive allergens found in foods, latex, and pollen grains. The cjp-8 cDNA encodes a 165-amino acid polypeptide possessing the conserved eight cysteines characteristic of plant LTP family members. Escherichia coli-expressed recombinant CJP-8 (r-CJP-8) reacted with IgE antibody from Japanese cedar pollinosis patients at a 37.5% frequency (6/16).

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