Generation of a Zinc Finger Protein ZPR1 Mutant That Constitutively Interacted with Translation Elongation Factor 1α

Noriyuki YANAKA; Yurika KASEDA; Anna TANAKA; Yoshihito NOGUSA; Naoki SUMIYOSHI; Norihisa KATO

doi:10.1271/bbb.90745

Biochemistry & Molecular Biology Communication

Generation of a Zinc Finger Protein ZPR1 Mutant That Constitutively Interacted with Translation Elongation Factor 1α

Noriyuki YANAKA, Yurika KASEDA, Anna TANAKA, Yoshihito NOGUSA, Naoki SUMIYOSHI, Norihisa KATO

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Keywords: zinc finger protein ZPR1, eukaryotic translation elongation factor 1α, zinc ion

JOURNAL FREE ACCESS

2009 Volume 73 Issue 12 Pages 2809-2811

DOI https://doi.org/10.1271/bbb.90745

View "Advance Publication" version (December 7, 2009).

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Abstract

Zinc finger protein ZPR1 (ZPR1) binds to eukaryotic translation elongation factor 1α (eEF1α) in response to growth stimuli, and is also involved in transcription and cell cycle regulation. In this study, we characterized the interaction of ZPR1 and eEF1α and generated a ZPR1 mutant that constitutively interacted with eEF1α. ZPR1-ΔA (Δ193–246) bound to eEF1α independently of Zn²⁺ in vivo. This study indicates that ZPR1-ΔA (Δ193–246) is a useful tool to provide structural insights into ZPR1 and to investigate the biological significance of the interaction between ZPR1 and eEF1α.

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