Surface-Displayed Expression of a Neutralizing Epitope of ApxIIA Exotoxin in Saccharomyces cerevisiae and Oral Administration of It for Protective Immune Responses against Challenge by Actinobacillus pleuropneumoniae

Jung-Mi KIM; Dea-Im JUNG; Yoo Jeong EOM; Seung-Moon PARK; Han-Sang YOO; Yong-Suk JANG; Moon-Sik YANG; Dae-Hyuk KIM

doi:10.1271/bbb.90941

Biochemistry & Molecular Biology Regular Papers

Surface-Displayed Expression of a Neutralizing Epitope of ApxIIA Exotoxin in Saccharomyces cerevisiae and Oral Administration of It for Protective Immune Responses against Challenge by Actinobacillus pleuropneumoniae

Jung-Mi KIM, Dea-Im JUNG, Yoo Jeong EOM, Seung-Moon PARK, Han-Sang YOO, Yong-Suk JANG, Moon-Sik YANG, Dae-Hyuk KIM

Author information

Jung-Mi KIM
Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University
Dea-Im JUNG
Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University
Yoo Jeong EOM
Korea Minjok Leadership Academy
Seung-Moon PARK
Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University
Han-Sang YOO
College of Veterinary Medicine, KRF Zoonotic Disease Priority Research Institute and BK21 for Veterinary Science, Seoul National University
Yong-Suk JANG
Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University
Moon-Sik YANG
Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University
Dae-Hyuk KIM
Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University

Keywords: surface display, APX toxin, Actinobacillus pleuropneumoniae, Saccharomyces cerevisiae, oral vaccination

JOURNAL FREE ACCESS

2010 Volume 74 Issue 7 Pages 1362-1367

DOI https://doi.org/10.1271/bbb.90941

View "Advance Publication" version (July 7, 2010).

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Abstract

A neutralizing epitope fragment of ApxIIA toxin (ApxIIA#5) of the Korean Actinobacillus pleuropneumoniae serotype 2 strain was expressed and immobilized on the cell surface of Saccharomyces cerevisiae for efficient vaccine development. Expression of ApxIIA#5 was confirmed by Western blot analysis using cell-wall proteins, and the surface display of ApxIIA#5 was further visualized under confocal microscopy. Quantitative ELISA revealed that the recombinant ApxIIA#5 directed to the cell surface consisted of approximately 16% cell-wall proteins, estimated to be 35 mg of ApxIIA#5 protein per liter of cultured cells. An immunoassay revealed that antigen-specific antibodies against ApxIIA#5 were present in the sera of mice fed recombinant ApxIIA#5-expressing yeast, but not in mice fed the wild-type nor the vector-only transformant. Moreover, the mice fed the recombinant epitope-expressing yeast were protected from injection of a lethal dose of A. pleuropneumoniae.

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