Vinegar Intake Enhances Flow-Mediated Vasodilatation via Upregulation of Endothelial Nitric Oxide Synthase Activity

Syoji SAKAKIBARA; Ryuichiro MURAKAMI; Mikio TAKAHASHI; Takashi FUSHIMI; Toyoaki MUROHARA; Mikiya KISHI; Yoshitaka KAJIMOTO; Masafumi KITAKAZE; Takayuki KAGA

doi:10.1271/bbb.90953

Food & Nutrition Science Regular Papers

Vinegar Intake Enhances Flow-Mediated Vasodilatation via Upregulation of Endothelial Nitric Oxide Synthase Activity

Syoji SAKAKIBARA, Ryuichiro MURAKAMI, Mikio TAKAHASHI, Takashi FUSHIMI, Toyoaki MUROHARA, Mikiya KISHI, Yoshitaka KAJIMOTO, Masafumi KITAKAZE, Takayuki KAGA

Author information

Syoji SAKAKIBARA
Central Research Institute, Mizkan Group Corporation
Ryuichiro MURAKAMI
Department of Cardiology, Nagoya University Graduate School of Medicine
Mikio TAKAHASHI
Central Research Institute, Mizkan Group Corporation
Takashi FUSHIMI
Central Research Institute, Mizkan Group Corporation
Toyoaki MUROHARA
Department of Cardiology, Nagoya University Graduate School of Medicine
Mikiya KISHI
Central Research Institute, Mizkan Group Corporation
Yoshitaka KAJIMOTO
Soiken Incorporation
Masafumi KITAKAZE
Cardiovascular Division of Medicine, National Cardiovascular Center
Takayuki KAGA
Central Research Institute, Mizkan Group Corporation

Keywords: acetic acid, endothelial function, endothelial nitric oxide synthase (eNOS) phosphorylation, AMP-activated protein kinase (AMPK), PKA

JOURNAL FREE ACCESS

2010 Volume 74 Issue 5 Pages 1055-1061

DOI https://doi.org/10.1271/bbb.90953

View "Advance Publication" version (May 7, 2010).

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Abstract

This study examined the effect of acetate on endothelial nitric oxide synthase (eNOS) in human umbilical vein endothelial cells (HUVECs) by immunoblotting assay and the ability of acetic acid to upregulate flow-mediated vasodilatation in humans. In HUVECs, acetate induced a biphasic increase in the phosphorylated form of eNOS. The amount of phosphorylated eNOS was significantly increased by exposure to 200 μmol/l acetate for 20 min (early phase) and for 4 h (late phase). The inhibitors of cAMP-dependent protein kinase (PKA) and AMP-activated protein kinase (AMPK) blocked acetate-induced eNOS phosphorylation in the early and the late phase respectively. Furthermore, in postmenopausal women, maximum forearm blood flow (FBF) in response to shear stress increased in the vinegar (acetic acid) administered group compared to the placebo group. These results suggest that acetic acid-induced eNOS phosphorylation contributes to upregulation of flow-mediated vasodilatation in humans.

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