Laboratory experiments to determine the effects of seeds collected from different parts of the plant on the seed quality of the lentil were carried out from August to September in 2004 and 2005 at the Seed Technology Laboratory, Bangladesh Agricultural Research Institute in Gazipur, Bangladesh. Lentil seeds of three varieties were harvested from different parts of the plant i.e. P₁: collection of pods from upper parts of lentil plants, P₂: collection of pods from middle parts of lentil plants, P₃: collection of pods from lower parts of lentil plants prior to harvesting. All the seeds were stored in earthen pot until conducting the laboratory study. A significant variation was observed in the three varieties of lentil for most of the parameters studied. The highest germination percentage, root length, shoot length, root plus shoot length and vigour were observed in the BARI Masur-4, and the lowest in the BARI Masur-2. Seeds collected from different parts had significant effect on some parameters studied. Collection of pods from middle parts of the lentil plants (P₂) recorded the highest germination percentage, root length, shoot length, root plus shoot length and vigour and the lowest moisture percentage. The BARI Masur-4 seeds collected from middle parts of lentil recorded the highest shoot length, root plus shoot length and vigour.