Effects of R-(−)-BPAP on the Expressions of Neurotrophins and Their Receptors in Mesencephalic Slices

Chieko Hirami; Kazue Takahata; Seiichiro Shimazu; Fumio Yoneda; Kyozo Hayashi; Hiroshi Katsuki; Akinori Akaike

doi:10.1248/bpb.28.1524

Notes

Effects of R-(−)-BPAP on the Expressions of Neurotrophins and Their Receptors in Mesencephalic Slices

Chieko Hirami, Kazue Takahata, Seiichiro Shimazu, Fumio Yoneda, Kyozo Hayashi, Hiroshi Katsuki, Akinori Akaike

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Keywords: R-(−)-1-(benzofuran-2-yl)-2-propylaminopentane, mesencephalon, brain-derived neurotrophic factor, trkB, slice culture, Parkinson's disease

JOURNAL FREE ACCESS

2005 Volume 28 Issue 8 Pages 1524-1526

DOI https://doi.org/10.1248/bpb.28.1524

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Abstract

R-(−)-1-(Benzofuran-2-yl)-2-propylaminopentane [R-(−)-BPAP] enhances electric field stimulation-induced release of catecholamine from isolated brain stem and ameliorates motor deficits in rats. We evaluated the effects of R-(−)-BPAP on the expression of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), and their receptors, trkB and p75^NTR in rat mesencephalic slice cultures. Levels of mRNA and protein were measured at 48 h after R-(−)-BPAP treatment by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. R-(−)-BPAP significantly increased the mRNA and protein levels of BDNF, without affecting the level of NT-3 mRNA. In addition, R-(−)-BPAP significantly increased the mRNA level of trkB, but not that of p75^NTR. These effects of R-(−)-BPAP may result in enhanced BDNF/trkB signaling, and could thus underlie the potential neurotrophic and antidepressant actions of this drug.

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