Virus entry, assembly, and budding are important processes in the replication cycle of a virus. Viruses are dependent on host living cells for their replication. Viruses use the proliferative mechanism of host cells for replication of viral components. Lipid rafts, specific membrane microdomains play a critical role in virus replication because localizing and concentrating viral components in the microdomains for entry, assembly, and budding of various types of virus. In this review, we describe the involvement of membrane lipid rafts in the virus replication cycle with our current findings for understanding the role of membrane lipid rafts in virus infection.