Beta-Mannosyl Linkages Negatively Regulate Anaphylaxis and Vasculitis in Mice, Induced by CAWS, Fungal PAMPs Composed of Mannoprotein–Beta-Glucan Complex Secreted by Candida albicans

Hiroyasu Shinohara; Noriko Nagi-Miura; Ken-ichi Ishibashi; Yoshiyuki Adachi; Akiko Ishida-Okawara; Toshiaki Oharaseki; Kei Takahashi; Shiro Naoe; Kazuo Suzuki; Naohito Ohno

doi:10.1248/bpb.29.1854

Regular Articles

Beta-Mannosyl Linkages Negatively Regulate Anaphylaxis and Vasculitis in Mice, Induced by CAWS, Fungal PAMPs Composed of Mannoprotein–Beta-Glucan Complex Secreted by Candida albicans

Hiroyasu Shinohara, Noriko Nagi-Miura, Ken-ichi Ishibashi, Yoshiyuki Adachi, Akiko Ishida-Okawara, Toshiaki Oharaseki, Kei Takahashi, Shiro Naoe, Kazuo Suzuki, Naohito Ohno

Author information

Hiroyasu Shinohara
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science
Noriko Nagi-Miura
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science
Ken-ichi Ishibashi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science
Yoshiyuki Adachi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science
Akiko Ishida-Okawara
Department of Bioactive Molecules, National Institute of Infectious Diseases
Toshiaki Oharaseki
Department of Pathology, Ohashi Hospital, Toho University School of Medicine
Kei Takahashi
Department of Pathology, Ohashi Hospital, Toho University School of Medicine
Shiro Naoe
Department of Pathology, Ohashi Hospital, Toho University School of Medicine
Kazuo Suzuki
Department of Bioactive Molecules, National Institute of Infectious Diseases
Naohito Ohno
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science

Keywords: Candida albicans, arteritis, mannoprotein, beta-1,2-mannan, DBA/2 mice

JOURNAL FREE ACCESS

2006 Volume 29 Issue 9 Pages 1854-1861

DOI https://doi.org/10.1248/bpb.29.1854

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Abstract

Candida albicans water soluble fraction (CAWS) is a water-soluble extracellular mannoprotein–beta-glucan complex obtained from the culture supernatant of Candida albicans, which grows in a chemically defined medium. CAWS induced toxic reactions, such as acute anaphylactoid reaction, by intravenous administration and coronary arteritis by intraperitoneal administration. To clarify the structure responsible for these toxic reactions, C. albicans was cultured in pH- and temperature-controlled conditions and prepared with CAWS with or without the beta-1,2-linked mannosyl segment (BM). The structure of CAWS was assessed by immunochemical and spectroscopic methodologies, and we found that CAWS prepared under the natural culture conditions contained only small amounts of BM and CAWS prepared at neutral conditions at 27 °C contained a significantly higher percentage of BM. Both the acute lethal toxicity and coronary arteritis induction was significantly more severe in the absence of BM. Activation of a complement pathway, the lectin pathway, by CAWS was significantly stronger in the absence of BM. These facts strongly suggest that BM linkages in CAWS negatively modulate acute and chronic toxicity of CAWS, and may be strongly related to the lectin pathway of the complement activation.

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