Purification of Liver Serine Protease Which Activates Microsomal Glutathione S-Transferase: Possible Involvement of Hepsin

Daisuke Kunii; Miyuki Shimoji; Shinji Nakama; Masashi Ikebe; Teruyuki Hachiman; Izumi Sato; Atsuko Tamaki; Kazuko Yamazaki; Yoko Aniya

doi:10.1248/bpb.29.868

Regular Articles

Purification of Liver Serine Protease Which Activates Microsomal Glutathione S-Transferase: Possible Involvement of Hepsin

Daisuke Kunii, Miyuki Shimoji, Shinji Nakama, Masashi Ikebe, Teruyuki Hachiman, Izumi Sato, Atsuko Tamaki, Kazuko Yamazaki, Yoko Aniya

Author information

Daisuke Kunii
Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
Miyuki Shimoji
Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
Shinji Nakama
Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
Masashi Ikebe
Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
Teruyuki Hachiman
Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
Izumi Sato
Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
Atsuko Tamaki
Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
Kazuko Yamazaki
Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus
Yoko Aniya
Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the Ryukyus

Keywords: glutathione S-transferase, hepsin, liver microsome, serine protease, activation

JOURNAL FREE ACCESS

2006 Volume 29 Issue 5 Pages 868-874

DOI https://doi.org/10.1248/bpb.29.868

Details

Abstract

Rat liver microsomal glutathione S-transferase (MGST1) is known to be activated by trypsin, however, it has not been clarified whether MGST1 is activated by a protease present in liver. In the present study we purified the MGST1 activating protease from liver microsomes and finally identified that the protease is hepsin, a type II transmembrane serine protease. When the protease was incubated with the purified MGST1 or liposomal MGST1 at 4 °C, MGST1 activity was increased 3—4.5 fold after 3—6 d. In electrophoretic and immunoblot analyses after the incubation of MGST1 with the protease MGST1 dimer and its degraded fragment were detected. These results suggest that the rat liver microsomal hepsin functions as MGST1 activating/degrading enzyme.

Corresponding author

Register with J-STAGE for free!