Inhibition of LPS-Induced iNOS, COX-2 and Cytokines Expression by Poncirin through the NF-κB Inactivation in RAW 264.7 Macrophage Cells

Jong-Bin Kim; Ah-Reum Han; Eun-Young Park; Ji-Yeon Kim; Woong Cho; Jun Lee; Eun-Kyoung Seo; Kyung-Tae Lee

doi:10.1248/bpb.30.2345

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Inhibition of LPS-Induced iNOS, COX-2 and Cytokines Expression by Poncirin through the NF-κB Inactivation in RAW 264.7 Macrophage Cells

Jong-Bin Kim, Ah-Reum Han, Eun-Young Park, Ji-Yeon Kim, Woong Cho, Jun Lee, Eun-Kyoung Seo, Kyung-Tae Lee

Author information

Jong-Bin Kim
Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University
Ah-Reum Han
College of Pharmacy and Center for Cell Signaling & Drug Discovery Research, Ewha Womans University
Eun-Young Park
Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University
Ji-Yeon Kim
Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University
Woong Cho
Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University
Jun Lee
College of Pharmacy and Center for Cell Signaling & Drug Discovery Research, Ewha Womans University
Eun-Kyoung Seo
College of Pharmacy and Center for Cell Signaling & Drug Discovery Research, Ewha Womans University
Kyung-Tae Lee
Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University

Keywords: poncirin, Poncirus trifoliata, inducible nitric oxide synthase, cyclooxygenase-2, nuclear factor-κB, anti-inflammation

JOURNAL FREE ACCESS

2007 Volume 30 Issue 12 Pages 2345-2351

DOI https://doi.org/10.1248/bpb.30.2345

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Abstract

We previously reported that poncirin, a flavanone glycoside isolated from the EtOAc extract of the dried immature fruits of Poncirus trifoliata, is an anti-inflammatory compound that inhibits PGE₂ and IL-6 production. The present work was undertaken to investigate the molecular actions of poncirin in RAW 264.7 macrophage cell line. Poncirin reduced lipopolysaccharide (LPS)-induced protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and the mRNA expressions of iNOS, COX-2, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in a concentration-dependent manner, as determined by Western blotting and RT-PCR, respectively. Furthermore, poncirin inhibited the LPS-induced DNA binding activity of nuclear factor-κB (NF-κB). Moreover, this effect was accompanied by a parallel reduction in IκB-α degradation and phosphorylation that in by nuclear translocations of p50 and p65 NF-κB subunits. Taken together, our data indicate that anti-inflammatory properties of poncirin might be the result from the inhibition iNOS, COX-2, TNF-α and IL-6 expression via the down-regulation of NF-κB binding activity.

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