Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Notes
Ephedrae Herba, a Component of Japanese Herbal Medicine Mao-to, Efficiently Activates the Replication of Latent Human Immunodeficiency Virus Type 1 (HIV-1) in a Monocytic Cell Line
Toru MurakamiHideki HaradaMary Ann SuicoTsuyoshi ShutoShinya SuzuHirofumi KaiSeiji Okada
Author information
JOURNAL FREE ACCESS

2008 Volume 31 Issue 12 Pages 2334-2337

Details
Abstract

The persistence of latent human immunodeficiency virus type 1 (HIV-1)-infected cellular reservoirs, despite prolonged treatment with highly active antiretroviral therapy (HAART), represents a major hurdle to virus eradication. In this study, we evaluated the effect of Japanese herbal medicine on the induction of HIV-1 replication in latently infected monocytic cell line, U1, in order to eradicate virus efficiently. We found that Mao-to was able to induce HIV-1 replication either alone or in combination with tumor necrosis factor-alpha (TNF-α). Among the four components of Mao-to, only Ephedrae herba had strong effects in inducing HIV-1 replication. Analysis by Western blotting revealed that Ephedrae herba induced the nuclear translocation of nuclear factor-kappa B (NF-κB). Reporter assay data also showed that Ephedrae herba and, slightly, Mao-to activated the NF-κB promoter, indicating that these herbal agents may induce HIV-1 replication through NF-κB activation. These findings suggest that Mao-to and its component, Ephedrea herba, may be good candidates to augment HAART by inducing the expression of latent HIV-1 with the ultimate goal of eliminating persistent viral reservoirs in individuals infected with HIV-1.

Content from these authors
© 2008 The Pharmaceutical Society of Japan
Previous article Next article
feedback
Top