The Anti-oxidative and Anti-inflammatory Effects of Caffeoyl Derivatives from the Roots of Aconitum koreanum R. R<small>AYMOND</small>

Kwan Hee Park; Min Park; Sun Eun Choi; Mi Sook Jeong; Joo Hee Kwon; Myung Hwan Oh; Hyung Kyun Choi; Seong Jun Seo; Min Won Lee

doi:10.1248/bpb.32.2029

Regular Articles

The Anti-oxidative and Anti-inflammatory Effects of Caffeoyl Derivatives from the Roots of Aconitum koreanum R. RAYMOND

Kwan Hee Park, Min Park, Sun Eun Choi, Mi Sook Jeong, Joo Hee Kwon, Myung Hwan Oh, Hyung Kyun Choi, Seong Jun Seo, Min Won Lee

Author information

Keywords: Aconitum koreanum, dicaffeoylquinic acid, anti-inflammatory activity, anti-oxidative activity

JOURNAL FREE ACCESS

2009 Volume 32 Issue 12 Pages 2029-2033

DOI https://doi.org/10.1248/bpb.32.2029

Details

Abstract

Activity guided fractionation of Aconitum koreanum root extract (RAK), a traditional medicine in Korea, afforded four caffeoyl derivatives, caffeic acid (1), 4,5-dicaffeoylquinic acid (2), 3,5-dicaffeoylquinic acid (3) and 3,5-dicaffeoylquinic acid methyl ester (4). In order to evaluate the anti-oxidative and anti-inflammatory effects of these compounds, their 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities and abilities to inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 were examined. And the protein and mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated HaCaT cells were also quantified by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR). Compounds (1—4) showed potent DPPH radical scavenging and NO inhibitory activities as compared with positive controls (L-ascorbic acid and N(G)-monomethyl-L-arginine (L-NMMA), respectively). Also, these compounds dose-dependently inhibited the expressions of iNOS and COX-2 as well as their mRNA levels.

Corresponding author

Register with J-STAGE for free!