Establishment of an in Vitro Peyer's Patch Cell Culture System Correlative to in Vivo Study Using Intestine and Screening of Lactic Acid Bacteria Enhancing Intestinal Immunity

Hekui Jin; Fumiko Higashikawa; Masafumi Noda; Xingrong Zhao; Yasuyuki Matoba; Takanori Kumagai; Masanori Sugiyama

doi:10.1248/bpb.33.289

Regular Articles

Establishment of an in Vitro Peyer's Patch Cell Culture System Correlative to in Vivo Study Using Intestine and Screening of Lactic Acid Bacteria Enhancing Intestinal Immunity

Hekui Jin, Fumiko Higashikawa, Masafumi Noda, Xingrong Zhao, Yasuyuki Matoba, Takanori Kumagai, Masanori Sugiyama

Author information

Hekui Jin
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University
Fumiko Higashikawa
Department of Molecular Nutrition Sciences, Graduate School of Biomedical Sciences, Hiroshima University
Masafumi Noda
Department of Molecular Nutrition Sciences, Graduate School of Biomedical Sciences, Hiroshima University
Xingrong Zhao
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University
Yasuyuki Matoba
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University
Takanori Kumagai
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University
Masanori Sugiyama
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University

Keywords: lactic acid bacterium, intestinal immunity, immunoglobulin A, probiotic

JOURNAL FREE ACCESS

2010 Volume 33 Issue 2 Pages 289-293

DOI https://doi.org/10.1248/bpb.33.289

Details

Abstract

Some lactic acid bacteria (LAB) are known as representative of probiotics. To screen LAB effective to enhance intestinal immunity, in the present study, we developed an accurate and convenient in vitro evaluation system using Peyer's patch cells (PP-cells) isolated from the mice intestine. We observed that the amount of immunoglobulin A (IgA) produced by PP-cells co-cultured with LAB was well correlative to that in PP-cells, intestine and feces isolated from live mice after oral administration of LAB [correlation coefficient (r)=0.888, 0.883, and 0.920, respectively]. In addition, using this in vitro system, we suggest that the IgA level of PP-cells co-culturing with plant-derived LAB might be more enhanced than with animal-derived LAB.

Corresponding author

Register with J-STAGE for free!