The Antinociceptive and Anti-inflammatory Action of the CHCl3-Soluble Phase and Its Main Active Component, Damnacanthal, Isolated from the Root of Morinda citrifolia

Kanako Okusada; Kazuo Nakamoto; Mikako Nishida; Wakako Fujita-Hamabe; Kohei Kamiya; Yoshiyuki Mizushina; Toshiko Satake; Shogo Tokuyama

doi:10.1248/bpb.34.103

Regular Articles

The Antinociceptive and Anti-inflammatory Action of the CHCl₃-Soluble Phase and Its Main Active Component, Damnacanthal, Isolated from the Root of Morinda citrifolia

Kanako Okusada, Kazuo Nakamoto, Mikako Nishida, Wakako Fujita-Hamabe, Kohei Kamiya, Yoshiyuki Mizushina, Toshiko Satake, Shogo Tokuyama

Author information

Kanako Okusada
Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kobe Gakuin University
Kazuo Nakamoto
Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kobe Gakuin University
Mikako Nishida
Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kobe Gakuin University
Wakako Fujita-Hamabe
Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kobe Gakuin University
Kohei Kamiya
Department of Pharmacognosy and Natural Product Chemistry, School of Pharmaceutical Sciences, Kobe Gakuin University
Yoshiyuki Mizushina
Laboratory of Food and Nutritional Sciences, Department of Nutritional Sciences, Kobe Gakuin University
Toshiko Satake
Department of Pharmacognosy and Natural Product Chemistry, School of Pharmaceutical Sciences, Kobe Gakuin University
Shogo Tokuyama
Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kobe Gakuin University

Keywords: Morinda citrifolia, damnacanthal, histamine H₁ receptor, antinociception, anti-inflammatory

JOURNAL FREE ACCESS

2011 Volume 34 Issue 1 Pages 103-107

DOI https://doi.org/10.1248/bpb.34.103

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Abstract

Morinda citrifolia (Rubiaceae, Noni) is a traditional medicine with various pharmacological activities. We investigated if the MeOH-, CHCl₃- and BuOH-soluble phase and its main active component, damnacanthal, isolated from the Noni root, have antinociceptive and anti-inflammatory actions in mice. The CHCl₃-soluble phase (3 g/kg, per os (p.o.)) significantly reduced pain-related behavior observed in the formalin test. These effects were not suppressed by pretreatment with naloxone (1 mg/kg, intraperitoneally (i.p.)), an opioid receptor antagonist. The CHCl₃-soluble phase (3 g/kg, p.o.) significantly reduced histamine-induced paw edema. The MeOH- and BuOH-soluble phase had no effect in either test. Furthermore, damnacanthal (10—100 mg/kg, p.o.) exerted an antinociceptive effect on chemical nociceptive stimuli, and decreased histamine-induced paw edema. Damnacanthal was weakly bound to the histamine H₁ receptor. These data suggest that the CHCl₃-soluble phase of the Noni root has antinociceptive and anti-inflammatory effects. Furthermore, these effects of damnacanthal isolated from the Noni root is mediated in part by the histamine H₁ receptor.

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