Hyperbaric Oxygen Induces Basic Fibroblast Growth Factor and Hepatocyte Growth Factor Expression, and Enhances Blood Perfusion and Muscle Regeneration in Mouse Ischemic Hind Limbs

Tetsuichi Asano; Eiji Kaneko; Shohei Shinozaki; Yutaka Imai; Masaharu Shibayama; Tsuyoshi Chiba; Masumi Ai; Akio Kawakami; Hiroshi Asaoka; Toru Nakayama; Yoshihiro Mano; Kentaro Shimokado

doi:10.1253/circj.71.405

Experimental Investigation

Hyperbaric Oxygen Induces Basic Fibroblast Growth Factor and Hepatocyte Growth Factor Expression, and Enhances Blood Perfusion and Muscle Regeneration in Mouse Ischemic Hind Limbs

Tetsuichi Asano, Eiji Kaneko, Shohei Shinozaki, Yutaka Imai, Masaharu Shibayama, Tsuyoshi Chiba, Masumi Ai, Akio Kawakami, Hiroshi Asaoka, Toru Nakayama, Yoshihiro Mano, Kentaro Shimokado

Author information

Tetsuichi Asano
Geriatrics and Vascular Medicine, Tokyo Medical and Dental University Graduate School
Eiji Kaneko
Geriatrics and Vascular Medicine, Tokyo Medical and Dental University Graduate School
Shohei Shinozaki
Geriatrics and Vascular Medicine, Tokyo Medical and Dental University Graduate School
Yutaka Imai
Geriatrics and Vascular Medicine, Tokyo Medical and Dental University Graduate School
Masaharu Shibayama
Occupational Health Education, Tokyo Medical and Dental University Graduate School
Tsuyoshi Chiba
Geriatrics and Vascular Medicine, Tokyo Medical and Dental University Graduate School
Masumi Ai
Geriatrics and Vascular Medicine, Tokyo Medical and Dental University Graduate School
Akio Kawakami
Geriatrics and Vascular Medicine, Tokyo Medical and Dental University Graduate School
Hiroshi Asaoka
Geriatrics and Vascular Medicine, Tokyo Medical and Dental University Graduate School
Toru Nakayama
Occupational Health Education, Tokyo Medical and Dental University Graduate School
Yoshihiro Mano
Occupational Health Education, Tokyo Medical and Dental University Graduate School
Kentaro Shimokado
Geriatrics and Vascular Medicine, Tokyo Medical and Dental University Graduate School

Keywords: Angiogenic growth factors, Angiogenesis, Hyperbaric oxygen, Ischemic limb, Skeletal muscle

JOURNAL FREE ACCESS

2007 Volume 71 Issue 3 Pages 405-411

DOI https://doi.org/10.1253/circj.71.405

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Abstract

Background It is not clear how hyperbaric oxygen therapy (HBO) affects ischemia-induced pathophysiological responses such as angiogenesis and skeletal muscle regeneration. In the present study the effects of HBO on the functional and morphological recovery of ischemic hind limbs, blood perfusion and the local production of angiogenic growth factors were studied in a mouse model. Methods and Results Mice were placed in pure oxygen under 3 atm for 1 h/day for 14 days after the removal of a segment of the left femoral artery. HBO-treated mice showed better functional recovery and greater blood flow in the ischemic hind limb than untreated mice. Histological examination revealed unatrophied muscle fibers with islands of small regenerating muscle cells only in HBO-treated mice. Regeneration of muscle was confirmed by the increase in myf5 mRNA. The amount of mRNA for vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF) and basic fibroblast growth factor (bFGF) was slightly increased in the ischemic hind limbs. HBO eliminated the increase in VEGF mRNA. In contrast, the amount of mRNA for bFGF and HGF was further increased by HBO treatment. HBO transiently increased early growth response protein 1 (Egr-1) in the ischemic hind limbs. Conclusions HBO accelerates the recovery of ischemic hind limbs by increasing the production of bFGF and HGF and by promoting muscle regeneration in mice. (Circ J 2007; 71: 405 - 411)

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