Delivery Route in Bone Marrow Cell Transplantation Should be Optimized According to the Etiology of Heart Disease

Hiroyuki Nakajima; Yutaka Sakakibara; Keiichi Tambara; Akira Marui; Momoko Yoshimoto; Goditha U. Premaratne; Xue Lin; Naoki Kanemitsu; Genichi Sakaguchi; Tadashi Ikeda; Kazunobu Nishimura; Tatsutoshi Nakahata; Masashi Komeda

doi:10.1253/circj.CJ-06-0430

Experimental Investigation

Delivery Route in Bone Marrow Cell Transplantation Should be Optimized According to the Etiology of Heart Disease

Hiroyuki Nakajima, Yutaka Sakakibara, Keiichi Tambara, Akira Marui, Momoko Yoshimoto, Goditha U. Premaratne, Xue Lin, Naoki Kanemitsu, Genichi Sakaguchi, Tadashi Ikeda, Kazunobu Nishimura, Tatsutoshi Nakahata, Masashi Komeda

Author information

Hiroyuki Nakajima
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University
Department of Cardiovascular Surgery, Mitsubishi Kyoto Hospital
Yutaka Sakakibara
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University
Keiichi Tambara
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University
Akira Marui
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University
Momoko Yoshimoto
Department of Pediatrics, Graduate School of Medicine, Kyoto University
Goditha U. Premaratne
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University
Xue Lin
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University
Naoki Kanemitsu
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University
Genichi Sakaguchi
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University
Tadashi Ikeda
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University
Kazunobu Nishimura
Department of Cardiovascular Surgery, Tenri Hospital
Tatsutoshi Nakahata
Department of Pediatrics, Graduate School of Medicine, Kyoto University
Masashi Komeda
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University

Keywords: Bone marrow cells, Cardiomyopathy, Myocardial infarction, Transplantation

JOURNAL FREE ACCESS

2008 Volume 72 Issue 9 Pages 1528-1535

DOI https://doi.org/10.1253/circj.CJ-06-0430

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Abstract

Background Recent studies have revealed that bone marrow cell (BMC) transplantation is effective not only for myocardial infarction (MI), but also for dilated cardiomyopathy (DCM). However, the method of administering donor cells remains unknown, and may differ between MI and DCM. In the present study, intramyocardial (IM) injection and intravenous (IV) delivery of BMC were compared in each etiological model. Methods and Results MI was induced in 72 mice and DCM in another 36 mice by doxorubicin. BMCs were administered IV or IM in an acute MI (AMI), old MI (OMI) or DCM model. In the AMI model, left ventricular (LV) remodeling was reduced in both the IM- and IV-groups, but only in the IM-group in the OMI model. In the DCM model, the LV dimension of the IV-group was smaller than that of the IM-group. Histological examination showed that green fluorescent protein (GFP) cells were equally distributed in the infarct area of the IV- and IM-groups in AMI, and in the IM-group in the OMI model. In the DCM model, GFP cells were diffusely scattered throughout the ventricular wall in the IV-group, but were confined to the injection site in the IM-group. Conclusions In OMI, IM delivery of BMCs was more effective than IV; however, IV delivery was superior in DCM. Delivery route should be selected according to the etiology of heart disease to optimize the efficacy of BMC transplantation. (Circ J 2008; 72: 1528 - 1535)

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