2006 Volume 54 Issue 1 Pages 33-36
A simple and sensitive kinetic spectrophotometric method for the determination of perindopril in pharmaceutical preparations is described. The method is based on the interaction of drug with 1-chloro-2,4-dinitrobenzene (CDNB) in dimethylsulfoxide (DMSO) at 40±1 °C. The reaction is followed spectrophotometrically by measuring the rate of change of the absorbance at 420 nm. Under the optimized experimental conditions, the calibration curve showed a linear relationship over the concentration range of 20—140 μg/ml. The activation parameter such as Ea, ΔH*, ΔS* and ΔG* for this reaction were calculated and found to be 27.31 kJ/mol, 24.69 kJ/mol, −138.84 J/K/mol and 61.50 kJ/mol, respectively. The method has been successfully applied to the determination of perindopril in commercial dosage forms. Statistical comparison of the results with the Abdellatef's spectrophotometric method6) shows excellent agreement and indicates no significant difference between the methods compared in terms of accuracy and precision.