Development of a High Performance Liquid Chromatographic Method for Systematic Quantitative Analysis of Chemical Constituents in Rhubarb

Katsuko Komatsu; Yorinobu Nagayama; Ken Tanaka; Yun Ling; Purusotam Basnet; Meselhy Ragab Meselhy

doi:10.1248/cpb.54.941

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Development of a High Performance Liquid Chromatographic Method for Systematic Quantitative Analysis of Chemical Constituents in Rhubarb

Katsuko Komatsu, Yorinobu Nagayama, Ken Tanaka, Yun Ling, Purusotam Basnet, Meselhy Ragab Meselhy

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Keywords: Rheum, HPLC, RG-tannin, rhatannin, quantitative comparison

JOURNAL FREE ACCESS

2006 Volume 54 Issue 7 Pages 941-947

DOI https://doi.org/10.1248/cpb.54.941

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Abstract

HPLC methods for the systematic determination of 30 compounds in Rhei Rhizoma (rhubarb) were developed. Using a combination of mobile phase gradient conditions and UV detection at 280 nm, all 30 compounds were separated satisfactorily with low detection limits (0.05—2 μg/ml). The developed methods provided a reliable calibration curve for each compound. By adopting these methods, the determination of 30 compounds in three kinds of rhubarb samples, derived from Rheum tanguticum, R. palmatum and R. officinale, was achieved. The constituent pattern of each rhubarb was clearly characterized through the quantitative composition of 30 major constituents of rhubarb.

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