Traditional Processing Strongly Affects Metabolite Composition by Hydrolysis in Rehmannia glutinosa Roots

Wen-Te Chang; Young Hae Choi; Rob Van der Heijden; Meng-Shiou Lee; Ming-Kuem Lin; Hongwei Kong; Hye Kyong Kim; Robert Verpoorte; Thomas Hankemeier; Jan Van der Greef; Mei Wang

doi:10.1248/cpb.59.546

Regular Articles

Traditional Processing Strongly Affects Metabolite Composition by Hydrolysis in Rehmannia glutinosa Roots

Wen-Te Chang, Young Hae Choi, Rob Van der Heijden, Meng-Shiou Lee, Ming-Kuem Lin, Hongwei Kong, Hye Kyong Kim, Robert Verpoorte, Thomas Hankemeier, Jan Van der Greef, Mei Wang

Author information

Wen-Te Chang
School of Chinese Medicine Resources, China Medical University
Young Hae Choi
Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University
Rob Van der Heijden
Division of Analytical Biosciences, Leiden/Amsterdam Center for Drug Research, Centre for Medical Systems Biology
Meng-Shiou Lee
School of Chinese Medicine Resources, China Medical University
Ming-Kuem Lin
School of Chinese Medicine Resources, China Medical University
Hongwei Kong
Division of Analytical Biosciences, Leiden/Amsterdam Center for Drug Research, Centre for Medical Systems Biology
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences
Hye Kyong Kim
Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University
Robert Verpoorte
Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University
Thomas Hankemeier
Division of Analytical Biosciences, Leiden/Amsterdam Center for Drug Research, Centre for Medical Systems Biology
Jan Van der Greef
Division of Analytical Biosciences, Leiden/Amsterdam Center for Drug Research, Centre for Medical Systems Biology
SU BioMedicine
Mei Wang
SU BioMedicine
Department of Applied Plant Science, TNO Quality of Life

Keywords: Rehmannia glutinosa, traditional processing, Fourier-transform-mass spectrometry, principle component analysis

JOURNAL FREE ACCESS

2011 Volume 59 Issue 5 Pages 546-552

DOI https://doi.org/10.1248/cpb.59.546

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Abstract

The processing of biological raw materials is considered to have an important role in the therapeutic application in Traditional Chinese Medicine. The root of Rehmannia glutinosa has to be processed by nine cycles of rice wine immersing, steaming and drying before using in clinical applications. In order to understand the chemical changes resulting from the processing, a comprehensive analysis of Rehmanniae radix was made using ¹H-NMR and Fourier transform (FT)-mass spectrometry in combination with multivariate data analysis. After ¹H-NMR and principle component analysis, hydrolysis was found to be the major chemical process during the treatments. Catalpol, raffinose and stachyose levels gradually decreased during processing, whereas monosaccharides including galactose and glucose were found to be higher in processed roots. The metabolic profile changed gradually through the processing cycles although the differences became smaller after the fifth processing cycle. The positive and negative ion-mode mass spectra by high resolution FT-MS revealed several series of ion clusters with mass differences of 162.053 Da, indicating a difference of a hexose moiety. During the processing, the number and signal intensity of the smaller glycosides were increased. Therefore, these results indicate that the fresh Rehmanniae radix is rich in polysaccharides, which are hydrolyzed during the processing.

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