We evaluated the concentration of fluoride release at the initial stage and after recharging the fluoride from a resin-based sealant containing surface reaction-type pre-reacted glass-ionomer (S-PRG) filler and conventional resin-based sealants. The concentration of other ions released from the sealants in the surrounding distilled water was also examined. At the initial stage, fluoride was released from all sealants. For the sealant containing S-PRG filler, a significantly higher amount of Sr and B ions was detected relative to those from other sealants. After sealant samples were soaked in a sodium fluoride solution, a significant amount of fluoride and other ions was released from the sealant containing S-PRG filler. These findings suggest that sealants containing S-PRG filler enhance the enamel demineralization-inhibiting effect and the remineralization-promoting effect for long-term dental care.