Drug Metabolism and Pharmacokinetics
Online ISSN : 1880-0920
Print ISSN : 1347-4367
ISSN-L : 1347-4367
Regular Articles
Single Nucleotide Polymorphisms and Haplotypes of CYP1A2 in a Japanese Population
Akiko SOYAMA, Yoshiro SAITO, Nobumitsu HANIOKA, Keiko MAEKAWA, Kazuo KOMAMURA, Shiro KAMAKURA, Masafumi KITAKAZE, Hitonobu TOMOIKE, Kazuyuki UENO, Yu-ichi GOTO, Hideo KIMURA, Masaaki KATOH, Kenji SUGAI, Osamu SAITOH, Mitsuru KAWAI, Teiichi OHNUMA, Taisuke OHTSUKI, Chieko SUZUKI, Narihiro MINAMI, Naoyuki KAMATANI, Shogo OZAWA, Jun-ichi SAWADA
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Keywords: CYP1A2, SNP, haplotype, Japanese
JOURNAL FREE ACCESS

2005 Volume 20 Issue 1 Pages 24-33

[Correction] DMPK Vol.20 (2005) No.2 p.152
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Abstract

  In order to identify genetic polymorphisms and haplotype frequencies of CYP1A2 in a Japanese population, the enhancer and promoter regions, all the exons with their surrounding introns, and intron 1 were sequenced from genomic DNA from 250 Japanese subjects. Thirty-three polymorphisms were found, including 13 novel ones: 2 in the enhancer region, 5 in the exons, and 6 in the introns. The most common single nucleotide polymorphism (SNP) was -163C>A (CYP1A2*1F allele) with a 0.628 frequency. In addition to six previously reported non-synonymous SNPs, three novel ones, 125C>G (P42R, CYP1A2*15 allele, MPJ6_1A2032), 1130G>A (R377Q, *16 allele, MPJ6_1A2033), and 1367G>A (R456H, *8 allele, MPJ6_1A2019), were found with frequencies of 0.002, 0.002, and 0.004, respectively. No polymorphism was found in the known nuclear transcriptional factor-binding sites in the enhancer region. Based on linkage disequilibrium analysis, the CYP1A2 gene was analyzed as one haplotype block. Using the 33 detected polymorphisms, 14 haplotypes were unambiguously identified, and 17 haplotypes were inferred by aid of an expectation-maximization-based program. Among them, the second major haplotype CYP1A2*1L is composed of -3860G>A (*1C allele), -2467delT (*1D allele), and -163C>A (*1F allele). Network analysis suggested that relatively rare haplotypes were derived from three major haplotypes, *1A, *1M, and *1N in most cases. Our findings provide fundamental and useful information for genotyping CYP1A2 in the Japanese, and probably Asian populations.

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© 2005 by The Japanese Society for the Study of Xenobiotics
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