Ion Channels on Silicon

S. J. Wilk; L. Petrossian; M. Goryll; T. J. Thornton; S. M. Goodnick; J. M. Tang; R. S. Eisenberg; M. Saraniti; D. Wong; J. J. Schmidt; C. D. Montemagno

doi:10.1380/ejssnt.2005.184

Conference -MB-ITR2005-

Ion Channels on Silicon

S. J. Wilk, L. Petrossian, M. Goryll, T. J. Thornton, S. M. Goodnick, J. M. Tang, R. S. Eisenberg, M. Saraniti, D. Wong, J. J. Schmidt, C. D. Montemagno

Author information

S. J. Wilk
Arizona State University, Center for Solid State Electronics Research, USA
L. Petrossian
Arizona State University, Center for Solid State Electronics Research, USA
M. Goryll
Arizona State University, Center for Solid State Electronics Research, USA
T. J. Thornton
Arizona State University, Center for Solid State Electronics Research, USA
S. M. Goodnick
Arizona State University, Center for Solid State Electronics Research, USA
J. M. Tang
Rush Medical College, Department of Molecular Biophysics and Physiology, USA
R. S. Eisenberg
Rush Medical College, Department of Molecular Biophysics and Physiology, USA
M. Saraniti
Illinois Institute of Technology, Department of Electrical and Computer Engineering, USA
D. Wong
UCLA, Department of Bioengineering, USA
J. J. Schmidt
UCLA, Department of Bioengineering, USA
C. D. Montemagno
UCLA, Department of Bioengineering, USA

Keywords: Biological molecules -proteins, Biomedical engineering, Ion channel, Lipid bilayer membrane, Microfabrication

JOURNAL FREE ACCESS

2005 Volume 3 Pages 184-189

DOI https://doi.org/10.1380/ejssnt.2005.184

Details

Abstract

We present results showing that silicon substrates can be used as a universal platform for recording the electrical activity of ion channels inserted into suspended bilayer membranes. The bilayers span 150 μm apertures etched into silicon substrates using standard microelectronics processing techniques. The silicon is oxidized, patterned with a 75 μm thick SU-8 epoxy resist and then coated with a thin layer of polytetrafluoroethylene rendering the surface hydrophobic. Reversible Ag/AgCl electrodes are integrated around the circumference of the opening and provide long-term stable measurements of the ion channel currents. Characteristic measurements of OmpF porin ion channel protein in phospholipid bilayers and α-hemolysin toxin protein in triblock copolymer layers were made. Long-term measurements showed that ion channel activity could be recorded 22 hours after initial formation of a lipid bilayer. [DOI: 10.1380/ejssnt.2005.184]

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