Fluorescence Recovery after Photo-bleaching Apparatus Using Second Harmonic of 1120 nm Semiconductor Laser for Illumination

Z. L. Zhang; T. Hara; H. Oguri; Y. J. Mo; M. Aoyama; H. Yoshida; Y. H. Kim; Md. M. Rahman; R. Tero; T. Urisu

doi:10.1380/ejssnt.2005.254

Conference -MB-ITR2005-

Fluorescence Recovery after Photo-bleaching Apparatus Using Second Harmonic of 1120 nm Semiconductor Laser for Illumination

Z. L. Zhang, T. Hara, H. Oguri, Y. J. Mo, M. Aoyama, H. Yoshida, Y. H. Kim, Md. M. Rahman, R. Tero, T. Urisu

Author information

Z. L. Zhang
The Graduate University for Advanced Studies, Japan
T. Hara
New Technology Research Laboratories, SUMITOMO OSAKA CEMENTO Co., Ltd., Japan
H. Oguri
New Technology Research Laboratories, SUMITOMO OSAKA CEMENTO Co., Ltd., Japan
Y. J. Mo
Physics Department, Henan University, China
Institute for Molecular Science, Japan
M. Aoyama
Institute for Molecular Science, Japan
H. Yoshida
Institute for Molecular Science, Japan
Y. H. Kim
The Graduate University for Advanced Studies, Japan
Md. M. Rahman
The Graduate University for Advanced Studies, Japan
R. Tero
Institute for Molecular Science, Japan
T. Urisu
The Graduate University for Advanced Studies, Japan
Institute for Molecular Science, Japan

Keywords: Semiconductor laser, Second harmonic generation, Fluorescence recovery after photo-bleaching, FRAP, Lipid, Fluorescence microscope

JOURNAL FREE ACCESS

2005 Volume 3 Pages 254-257

DOI https://doi.org/10.1380/ejssnt.2005.254

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Abstract

A compact fluorescence recovery after photo-bleaching (FRAP) apparatus was constructed using a semiconductor laser as an illumination light source. The second harmonic, 560 nm, of the 1120 nm semiconductor laser is guided to the light source point of the fluorescence microscope by an optical fiber. Since the illumination light wavelength 560 nm almost fits to the absorption maximum of the fluorescence dye molecule rhodamine B sulfonyl, the photo-bleaching was induced efficiently with minimum heating of the microscope lens system. The time dependence of the fluorescence recovery was directly measured by the photo-multiplier attached and the diffusion coefficient of the lipid molecules was calculated. [DOI: 10.1380/ejssnt.2005.254]

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