High-speed Atomic Force Microscopy for Capturing Dynamic Behavior of Protein Molecules at Work

Toshio Ando; Noriyuki Kodera; Takayuki Uchihashi; Atsushi Miyagi; Ryo Nakakita; Hayato Yamashita; Keiko Matada

doi:10.1380/ejssnt.2005.384

Conference -ISSS-4-

High-speed Atomic Force Microscopy for Capturing Dynamic Behavior of Protein Molecules at Work

Toshio Ando, Noriyuki Kodera, Takayuki Uchihashi, Atsushi Miyagi, Ryo Nakakita, Hayato Yamashita, Keiko Matada

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Keywords: Atomic force microscopy, biological aspects of nanostructures, proteins

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Supplementary material

2005 Volume 3 Pages 384-392

DOI https://doi.org/10.1380/ejssnt.2005.384

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Abstract

It seems that only the atomic force microscope (AFM) has a potential to trace a protein in action with high spatial and temporal resolution. To realize that potential, we have been developing a high-speed AFM. We have made efforts not only to enhance the scan speed but also to reduce the tip-sample interaction force as much as possible. The high-speed AFM can capture moving protein molecules and weakly interacting protein molecules on video without disturbing their physiological function. Here we review our studies carried out over the past 10 years, and our preliminary work toward the next generation of the instrument. [DOI: 10.1380/ejssnt.2005.384]

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