e-Journal of Surface Science and Nanotechnology
Online ISSN : 1348-0391
ISSN-L : 1348-0391
Conference -Nano-org. & Func.-
An artificial lipid bilayer formed on a PEG-coated glass for simultaneous electrical and optical measurement of single ion-channels
Toru IdeYuko TakeuchiTakaaki AokiKazuhito TabataHiroyuki Noji
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2005 Volume 3 Pages 70-73

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Abstract

The purpose of this study is to develop an apparatus for simultaneous measurement of electrical and spectroscopic parameters of single ion-channels. We have combined the single channel recording apparatus with an artificial lipid bilayer and a fluorescence microscope designed to detect single fluorescent molecules. The artificial membranes were formed on a PEG (polyethylene glycol)-coated glass and observed with an objective-type total internal reflection fluorescence (TIRF) microscope. The lateral motion of a single lipid molecule (β-BODIPY 530/550 HPC) was recorded. The lateral diffusion constant of the lipid molecule was calculated from the trajectories of single molecules as D=8.0± 4.0× 10-8 cm2/s. Ionic channels were incorporated into the membrane and single-channel current fluctuations were recorded. These data show the possibility of the present technique for simultaneous measurement of electrical and spectroscopic parameters of single-channel activities. [DOI: 10.1380/ejssnt.2005.70]

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この記事はクリエイティブ・コモンズ [表示 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by/4.0/deed.ja
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