e-Journal of Surface Science and Nanotechnology
Online ISSN : 1348-0391
ISSN-L : 1348-0391
Conference -ALC05-
Enhanced Peptide Molecular Imaging Using Sodium Salt Aqueous Droplets
M. KomatsuY. MurayamaK. KugeH. Hashimoto
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JOURNAL FREE ACCESS

2006 Volume 4 Pages 468-472

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Abstract

A new method, droplet-enhanced secondary ion mass spectrometry (SIMS), was developed for the molecular imaging analysis of biological samples. To facilitate the ionization of bio-molecules, a few microliters of aqueous solution containing sodium salt was dropped onto a peptide sample before time of flight (TOF)-SIMS measurement. Using this method, we have successfully obtained strong molecular secondary ion signals from molecular weight (MW) < 1587 peptide samples while maintaining their natural distribution. On the basis of various experiments, we observed that a neutral sputtered peptide molecule (M0) is effectively ionized by collision with a sodium ion (Na+) above the sample surface (M0 ← Na+), and excess sodium salt doped onto the sample has effect on inhibition of the positive ion formation. This method enables to simultaneously induce the ionization of each peptide present in a multicomponent solution. We also succeeded in detecting and quantifying nanogram levels of peptides with bubble jet (BJ) printing droplets. The results obtained indicate the usefulness of TOF-SIMS for micro-meter order sized peptide/protein screening system for assessing disease and drug efficacy. [DOI: 10.1380/ejssnt.2006.468]

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この記事はクリエイティブ・コモンズ [表示 4.0 国際]ライセンスの下に提供されています。
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