e-Journal of Surface Science and Nanotechnology
Online ISSN : 1348-0391
ISSN-L : 1348-0391
Conference -ICSFS-14-
Self Assemblage of Beta Sheeted Structures on Solid State Film for Biosensors
A. ŠetkusV. BukauskasS. PavliukS. BruzyteV. CasaiteR. Meškys
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2009 Volume 7 Pages 491-496

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Abstract

Based on modification of beta-sheeted protein structures in biosynthesis stage selfassemblage of 3D structures on solid state surfaces is investigated. A set of hybrid proteins containing the abeta40 peptide domain are constructed and characterized. The extended biomolecular structures are immobilised on the solid surfaces. Self assembled hybrid structures on the surface are obtained by deposition of the biomolecular conglomerates from buffer solutions. The surfaces of mica, SiO2, SnO and In2O3 are covered with the biomolecular layer. Formation of hybrid structures containing beta-sheeted fibrils is dependent on immobilisation period and origin of the solid surface. Morphology, mechanical and electrical properties are described by scanning probe microscope. Stabile fibrils of diameter about 2 nm and length of about 100-1000 nm are detected for lysozyme while Abeta40 peptide base proteins and TrxAbeta40 mutants result in specific structures. The biomolecular layers were described by analytical model based on digital representation of the surface profile data and analysis of the power spectral density function. [DOI: 10.1380/ejssnt.2009.491]

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この記事はクリエイティブ・コモンズ [表示 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by/4.0/deed.ja
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