e-Journal of Surface Science and Nanotechnology
Online ISSN : 1348-0391
ISSN-L : 1348-0391
Conference -ISSS-5-
SPR Sensor Chips with Polymer Nanogratings
Young-Ho ChoYoung-Hyun Jin
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2009 Volume 7 Pages 750-756

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Abstract

This paper presents low-cost, multi-channel, nano-grating SPR (Surface Plasmon Resonance) sensor chips for applications to disposable biomolecule detection. The present SPR sensor chip consists of two fluidic channels, including a sample channel and a reference channel, each having 833 nm-pitch PDMS gratings. The SPR signals from each channel are coupled by an external mirror and the SPR dip shift in the coupled signals is detected by a single spectrometer. Compared to the previous multi-channel SPR sensors using fiber-optic switches or special prisms, the present SPR sensor chips with PDMS nanogratings result in more compact and less expensive SPR detectors. We have designed and fabricated the SPR sensor chips with two channel gratings having the refractive index sensitivity of 321.78 nm/RI and 514.26 nm/RI, respectively. From the fabricated SPR sensor chips, we measure the SPR dip shift of 1.13±0.16 nm for the target biomolecules of 0.5 μM streptavidin binded on a 10% biotinylated surface. The real-time binding reaction rates of m-antibiotin has been monitored for the fixed concentration of 0.2 μM streptavidin as well as for the varying concentration range of 2-50 nM; thus obtaining the results for the fixed and varying concentration in good agreement with those obtained from a commercialized SPR sensor. It is demonstrated that the polymer nanograting SPR sensor chips offer simple and disposable biomolecule detection methods for point-of-care applications. [DOI: 10.1380/ejssnt.2009.750]

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この記事はクリエイティブ・コモンズ [表示 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by/4.0/deed.ja
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