Clinical Implications of Pre-Operative Rapid BRAF Analysis for Papillary Thyroid Cancer

Atsushi KUMAGAI; Hiroyuki NAMBA; Zhanay AKANOV; Vladimir A. SAENKO; Serik MEIRMANOV; Akira OHTSURU; Hiroshi YANO; Shigeto MAEDA; Masanobu ANAMI; Tomayoshi HAYASHI; Masahiro ITO; Sagadat SAGANDIKOVA; Zhanar ELEUBAEVA; Daniyal MUSSINOV; Maira ESPENBETOVA; Shunichi YAMASHITA

doi:10.1507/endocrj.K06-194

ORIGINALS

Clinical Implications of Pre-Operative Rapid BRAF Analysis for Papillary Thyroid Cancer

Atsushi KUMAGAI, Hiroyuki NAMBA, Zhanay AKANOV, Vladimir A. SAENKO, Serik MEIRMANOV, Akira OHTSURU, Hiroshi YANO, Shigeto MAEDA, Masanobu ANAMI, Tomayoshi HAYASHI, Masahiro ITO, Sagadat SAGANDIKOVA, Zhanar ELEUBAEVA, Daniyal MUSSINOV, Maira ESPENBETOVA, Shunichi YAMASHITA

Author information

Atsushi KUMAGAI
Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences
Department of Public Health and Environment, World Health Organization
Hiroyuki NAMBA
Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences
Zhanay AKANOV
Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences
Vladimir A. SAENKO
International Health and Radiation Research, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences
Serik MEIRMANOV
Tissue and Histopathology Section, Division of Scientific Data Registry, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences
Akira OHTSURU
Takashi Nagai Memorial International Hibakusha Medical Center, Nagasaki University Hospital
Hiroshi YANO
Division of Surgical Oncology, Department of Translational Medical Sciences, Nagasaki University Graduate School of Biomedical Sciences
Shigeto MAEDA
Division of Endocrine Surgery, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences
Masanobu ANAMI
Department of Clinical Pathology, Nagasaki University Hospital
Tomayoshi HAYASHI
Department of Clinical Pathology, Nagasaki University Hospital
Masahiro ITO
Department of Pathology, National Nagasaki Medical Center
Sagadat SAGANDIKOVA
Semipalatinsk Diagnostic Center
Zhanar ELEUBAEVA
Semipalatinsk Regional Oncology Center
Daniyal MUSSINOV
Semipalatinsk Regional Oncology Center
Semipalatinsk State Medical Academy
Maira ESPENBETOVA
Semipalatinsk State Medical Academy
Shunichi YAMASHITA
Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences
Department of Public Health and Environment, World Health Organization
International Health and Radiation Research, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences
Takashi Nagai Memorial International Hibakusha Medical Center, Nagasaki University Hospital

Keywords: Papillary thyroid carcinoma, BRAF, Mutation, Fine needle aspiration biopsy

JOURNAL FREE ACCESS

2007 Volume 54 Issue 3 Pages 399-405

DOI https://doi.org/10.1507/endocrj.K06-194

View "Advance Publication" version (April 12, 2007).

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Abstract

The activating point mutation of the BRAF gene, BRAF^T1799A, is the most common and specific genetic alteration in adult papillary thyroid carcinoma (PTC) and a possible marker of malignant potential of PTC. We have applied the PCR-RFLP method using fine-needle aspiration biopsy samples not only to our clinical practice but also to the international medical assistance effort around the Semipalatinsk Nuclear Testing Site in Kazakhstan. Seventy-seven cases (100 nodules) from Japan and 131 cases (137 nodules) from Kazakhstan were examined. There were 14 Japanese and 76 Kazakhstani cases of cytological malignant tumors from the examined samples. We detected 12 (85.7% of PTC) and 19 (25% of PTC) cases with BRAF^T1799A among the Japanese and Kazakhstani cases, respectively. Of these cases, we found mutations in one cytologically "suspicious" case and even in two pathologically "benign" cases (after surgery in Kazakhstan). All of the BRAF mutation-positive cases, including those three, were confirmed as PTC by careful pathological examination, including immunohistochemical analysis. In summary, our PCR-RFLP method for BRAF^T1799A detection using FNAB samples is useful not only for preoperative diagnosis of PTC but also as a complementary diagnostic tool for accurate pathological diagnosis, even after surgery.

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