Identification of Nocardia farcinica by a PCR Primer Amplifying a Specific DNA Band for the Bacterium

Taichi Hasegawa; Tohru Gonoi; Junji Ito; Takahisa Kogure; Katsukiyo Yazawa; Yuzuru Mikami

doi:10.3314/jjmm.48.173

Short Reports

Identification of Nocardia farcinica by a PCR Primer Amplifying a Specific DNA Band for the Bacterium

Taichi Hasegawa, Tohru Gonoi, Junji Ito, Takahisa Kogure, Katsukiyo Yazawa, Yuzuru Mikami

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Keywords: Nocardia farcinica, RAPD analysis, specific PCR primer, specific identification

JOURNAL FREE ACCESS

2007 Volume 48 Issue 4 Pages 173-175

DOI https://doi.org/10.3314/jjmm.48.173

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Abstract

A PCR primer specific to Nocardia farcinica was prepared based on sequence information of random amplified polymorphic DNA (RAPD) analysis. The PCR primer amplifies N. farcinica species only; no amplification was observed in 25 other Nocardia strains that we tested. Specificity of the primer for N. farcinica was also confirmed using other fungal and bacterial strains that are frequently isolated from clinical samples such as sputa and broncho alveolar lavage (VAL) .

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