Estrogens (estron: E1; estradiol: E2; estriol: E3) are major water pollutants. For the removal of estrogens, activated carbon (AC) and ozone treatments were employed, and the chemical oxygen demand (COD) and pH of a single solution and multiple solution systems were investigated. The removal of estrogens increased with the amount of AC. The percentage of removed estrogens by AC in the single solution system was greater than that in the ternary solution system. The estrogens were completely removed using ozone, which also reduced COD and pH. The removal of estrogens increased the water quality and decreased the amount of organic carbon.