Mild Electrical Stimulation Increases Ubiquitinated Proteins and Hsp72 in A549 Cells via Attenuation of Proteasomal Degradation

Saori Morino; Mary Ann Suico; Tatsuya Kondo; Erika Sekimoto; Shuichiro Yano; Tomoko Matsuda; Takashi Matsuno; Tsuyoshi Shuto; Eiichi Araki; Hirofumi Kai

doi:10.1254/jphs.08180SC

Short Communications

Mild Electrical Stimulation Increases Ubiquitinated Proteins and Hsp72 in A549 Cells via Attenuation of Proteasomal Degradation

Saori Morino, Mary Ann Suico, Tatsuya Kondo, Erika Sekimoto, Shuichiro Yano, Tomoko Matsuda, Takashi Matsuno, Tsuyoshi Shuto, Eiichi Araki, Hirofumi Kai

Author information

Saori Morino
Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Global COE “Cell Fate Regulation Research and Education Unit”, Kumamoto University, Japan
Mary Ann Suico
Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Global COE “Cell Fate Regulation Research and Education Unit”, Kumamoto University, Japan
Tatsuya Kondo
Department of Metabolic Medicine, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Japan
Erika Sekimoto
Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Global COE “Cell Fate Regulation Research and Education Unit”, Kumamoto University, Japan
Shuichiro Yano
Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Global COE “Cell Fate Regulation Research and Education Unit”, Kumamoto University, Japan
Tomoko Matsuda
Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Global COE “Cell Fate Regulation Research and Education Unit”, Kumamoto University, Japan
Takashi Matsuno
Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Global COE “Cell Fate Regulation Research and Education Unit”, Kumamoto University, Japan
Tsuyoshi Shuto
Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Global COE “Cell Fate Regulation Research and Education Unit”, Kumamoto University, Japan
Eiichi Araki
Department of Metabolic Medicine, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Japan
Hirofumi Kai
Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Global COE “Cell Fate Regulation Research and Education Unit”, Kumamoto University, Japan

Keywords: mild electrical stimulation, proteasomal degradation, ubiquitinated protein

JOURNAL FREE ACCESS

2008 Volume 108 Issue 2 Pages 222-226

DOI https://doi.org/10.1254/jphs.08180SC

View "Advance Publication" version (October 10, 2008).

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Abstract

To explore the cellular effects of mild electrical stimulation (MES), we treated A549 cells with low-intensity direct current at 5 V applied for 10 min. MES did not induce cell cytotoxicity or the unfolded protein response (UPR). Interestingly, the expression of ubiquitinated proteins and heat shock protein (Hsp) 72 was increased but not that of other Hsps. MES attenuated the degradation of Hsp72, which is a substrate of the proteasome-ubiquitin system. These results, along with the observed increase in expression of ubiquitinated proteins, imply that MES may affect the proteasome system, which regulates the fate of many proteins.

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