Expression of the Ubiquitin Ligase HRD1 in Neural Stem/Progenitor Cells of the Adult Mouse Brain

Koichi Kawada; Masayuki Kaneko; Yasuyuki Nomura; Seisuke Mimori; Hiroshi Hamana; Kiyokazu Ogita; Toshihiko Murayama; Hiromichi Fujino; Yasunobu Okuma

doi:10.1254/jphs.11120SC

Short Communications

Expression of the Ubiquitin Ligase HRD1 in Neural Stem/Progenitor Cells of the Adult Mouse Brain

Koichi Kawada, Masayuki Kaneko, Yasuyuki Nomura, Seisuke Mimori, Hiroshi Hamana, Kiyokazu Ogita, Toshihiko Murayama, Hiromichi Fujino, Yasunobu Okuma

Author information

Koichi Kawada
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Chiba Institute of Science, Japan
Masayuki Kaneko
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Chiba Institute of Science, Japan
Yasuyuki Nomura
Laboratory of Pharmacotherapeutics, Yokohama College of Pharmacy, Japan
Seisuke Mimori
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chiba Institute of Science, Japan
Hiroshi Hamana
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chiba Institute of Science, Japan
Kiyokazu Ogita
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Japan
Toshihiko Murayama
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University, Japan
Hiromichi Fujino
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University, Japan
Yasunobu Okuma
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Chiba Institute of Science, Japan

Keywords: HRD1, hippocampus, subventricular zone

JOURNAL FREE ACCESS

2011 Volume 117 Issue 3 Pages 208-212

DOI https://doi.org/10.1254/jphs.11120SC

View "Advance Publication" version (October 25, 2011).

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Abstract

Neural stem/progenitor cells (NSCs) reside in the subventricular zone (SVZ) and subgranular zone of the hippocampal dentate gyrus in adult mammals. The ubiquitin ligase HRD1 is associated with degradation of amyloid precursor protein and believed to be specifically expressed in neurons and not in astrocytes. We investigated expression of HRD1 using immunohistochemistry and found colocalization of HRD1 with the NSC marker protein nestin and glial fibrillary acidic protein in the NSCs of the SVZ (the SVZ astrocytes) but not in the hippocampus. In the hippocampal dentate gyrus, HRD1 is localized in the nucleus of nestin-positive cells.

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