Journal of Pharmacological Sciences
Online ISSN : 1347-8648
Print ISSN : 1347-8613
ISSN-L : 1347-8613
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Protective Effect of Buckwheat Polyphenols Against Long-Lasting Impairment of Spatial Memory Associated With Hippocampal Neuronal Damage in Rats Subjected to Repeated Cerebral Ischemia
Fengling PuKenichi MishimaNobuaki EgashiraKatsunori IwasakiTomohiro KanekoTomoko UchidaKeiichi IrieDaisuke IshibashiHajime FujiiKenichi KosunaMichihiro Fujiwara
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2004 Volume 94 Issue 4 Pages 393-402

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Abstract

In the present experiment, we studied the action of buckwheat polyphenol (BWP, from Fagopyrum esculentum MOENCH) in a repeated cerebral ischemia model, which induced a strong and long-lasting impairment of spatial memory in 8-arm radial maze with hippocampal CA1 cell death in rats. BWP (600 mg/kg, continuous 21-day p.o.) significantly ameliorated not only the impairment of spatial memory in the 8-arm radial maze, but also necrosis and TUNEL-positive cells in the hippocampal CA1 area subjected to repeated cerebral ischemia (10 min × 2 times occlusion, 1-h interval) in rats. In order to investigate the mechanism of BWP protective action, we measured the release of glutamate and NOx (NO2 + NO3) production induced by repeated cerebral ischemia in the rat dorsal hippocampus using microdialysis. A 14-day BWP treatment significantly inhibited the excess release of glutamate after the second occlusion. In addition, the BWP remarkably suppressed a delayed increase in NOx (NO2 + NO3) induced by repeated cerebral ischemia in the dorsal hippocampus as determined in vivo by microdialysis. However, the 14-day treatment did not affect hippocampal blood flow in either intact rats or rats subjected to repeated ischemia measured by lasser Doppler flowmeter. These results suggested that BWP might ameliorate spatial memory impairment by inhibiting glutamate release and the delayed generation of NOx in rats subjected to repeated cerebral ischemia.

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© The Japanese Pharmacological Society 2004
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