A dramatic increase in intracellular Ca²⁺ concentration ([Ca²⁺]_i) occurs in eggs at fertilization common to all animal species examined to date, and this serves as a pivotal signal for egg activation characterized by resumption of meiotic cell division and formation of the pronuclei. In mammalian eggs, repetitive [Ca²⁺]_i rises (Ca²⁺ oscillations) each of which accompanies a propagating wave across the egg occur due to release of Ca²⁺ from the endoplasmic reticulum mainly through type 1 inositol 1,4,5-trisphosphate (IP₃) receptor. Ca²⁺ oscillations are induced by a cytosolic sperm factor driven into the egg cytoplasm upon sperm-egg fusion. A current strong candidate of the sperm factor is a novel sperm-specific isozyme of phospholipase C (IP₃-producing enzyme), PLCζ. Recent extensive research has reveled characteristics of PLCζ such as the Ca²⁺ oscillation-inducing activity after injection of PLCζ-encoding RNA or recombinant PLCζ into mouse eggs, extremely high Ca²⁺-sensitivity of the enzymatic activity in vitro, and nuclear translocation ability possibly related to cell-cycle-dependent regulation of Ca²⁺ oscillations. [Ca²⁺]_i rises cause successive activation of calmodulin-dependent kinase II and E3 ubiquitin ligase, lead to proteolysis of ubiquitinated cyclin B1 and inactivation of metaphase-promoting factor (Cdk1/cyclin B1 complex), and result in the release of eggs from meiotic arrest.